Simultaneous Analysis of DNPH-Derivatized Aldehydes Using Integrated HPLC and C30 Column

User Benefits

- DNPH-derivatized aldehydes can be analyzed using i-Series LC-2070C integrated HPLC. - The six compounds designated under the Offensive Odor Control Law and the 15 compounds designated by EPA can be simultaneously analyzed. - Multiple isomers of DNPH-derivatized aldehydes can be separated using Shim-pack SR-C30 column.

Introduction

Aldehydes are the source of concern regarding environmental pollution and negative effects on human health, and analytical methods for aldehydes have been established. Analysis for formaldehyde and acetaldehyde, which are designated as harmful air pollutants, were presented in Application News No. 01-00996. This study presents the simultaneous determination of 16 carbonyl compounds, including six substances regulated under the Offensive Odor Control Law in Japan and 15 compounds listed by the U.S. Environmental Protection Agency (EPA), using a 2,4-dinitrophenylhydrazine (DNPH) derivatization coupled with high-performance liquid chromatography (HPLC). Analyses were performed using an i-Series LC-2070C integrated HPLC system, designed for robust routine operation. Chromatographic separation was achieved with a Shim-pack SR-C30 column featuring a triacontyl (C30) stationary phase. The C30 stationary phase provides enhanced shape selectivity and superior structural recognition, making it particularly suitable for the separation of highly lipophilic compounds as well as the effective resolution of structural isomers.

May 7, 2026 GMT