Simultaneous Analysis of Etizolam, Triazolam, and Their Metabolites in Biospecimens Using LCMS™-9030

Introduction

Etizolam and Triazolam are psychotropic drugs in the benzodiazepine class, which are used for a wide range of purposes due to their properties as a sedative, hypnotic drug, anxiolytic, anticonvulsant, and muscle relaxant. Benzodiazepines and their metabolites in the body are important analytes in the field of forensic toxicology and therefore more reliable toxicology test methods, such as liquid chromatography mass spectrometry (LC/MS), are needed. Since Etizolam, Triazolam, and their metabolites (alpha-Hydroxyetizolam, alpha-Hydroxytriazolam, and 4-Hydroxytriazolam) have similar structures or almost the same molecular weight, it is difficult to separate them using high performance liquid chromatography (HPLC) or by the difference in m/z values that can be detected by a quadrupole mass analyzer with nominal mass resolving power. In this experiment, we simultaneously analyzed Etizolam, Triazolam, and their metabolites using the Nexera X2 ultra high performance liquid chromatograph and LCMS-9030 high-resolution mass spectrometer.

April 3, 2019 GMT