Quantitation of Nucleic Acid Using BioSpec-nano

Introduction

The BioSpec-nano has 2 optical pathlengths (0.2 mm, 0.7 mm), allowing quantitation of nucleic acid with sample volumes of 1 μL and 2 μL, respectively. In addition, if the optional cell (sample volume: 2 mL) having a 5 mm pathlength is used, measurement can be conducted using the cell (cuvette). Here we conducted measurement of dsDNA at various concentrations at each optical pathlength, and we report the results on the linearity of photometric values (OD) obtained from nucleic acid quantitation, as well as the measurement repeatability as CV (%), and accuracy of the photometric values (OD). In addition, we also investigated the effect of the automatic wiping performance conducted during nucleic acid quantitation.

June 28, 2010 GMT