Micro Volume Photometric Measurement of Nucleic Acid using TrayCell with UV-1280

Introduction

Nucleic acid concentration can be determined easily using an ultraviolet-visible (UV-VIS) spectrophotometer. Such samples are typically available in small micro volume (less than 5 µl), and a system that is able to measure micro volume with high reproducibility will be preferred. A conventional 10 mm path length super micro cuvette requires at least 50 µl of sample and needs extra care in cleaning to prevent carryover. Hellma TrayCell is a fiber-optic ultra-micro cell designed for the UV-VIS micro volume analysis of nucleic acids and proteins. The sample volume requirement ranges from 0.7 µl to 10 µl depending on the path length used. Shimadzu UV-1280 offers a bio method mode as a standard function for DNA and protein concentrations determination.

May 15, 2015 GMT