Prominence Inert LC System
Inert LC System
Ideal for Size Exclusion Chromatography of Biopolymers
A new inert LC system is now part of Prominence series. Featuring the same high reliability, robustness, and expandability as the Prominence series, this system is ideal for the analysis of biopolymers such as antibody drugs.
The Prominence inert LC system employs non-metallic materials such as PEEK resin in the liquid contact parts for the mobile phase and sample solution. It not only enables high concentration salts and acids to be used in the mobile phase, but it also suppresses the adsorption of samples with high affinity to metals.
The newly designed LC-20Ai solvent delivery unit offers highly reproducible analyses due to an extremely accurate liquid delivery capability.
In size exclusion chromatography (SEC) analysis of biomolecules, a mobile phase containing halide salts such as sodium chloride is delivered to an SEC column in isocratic mode, the molecular weights are determined and aggregates such as dimers analyzed. In order to obtain stable data in SEC, a highly accurate solvent delivery capability is required.
Ion Exchange Chromatography
In ion exchange chromatography (IEX) analysis of biomolecules, a mobile phase containing halide salts is delivered to an IEX column in gradient mode and charge variants separated and detected. In order to obtain stable data in IEX, a highly accurate gradient delivery capability is required.
- Note: Monoclonal antibodies were provided by Assistant Professor Takashi Matsuzaki of the Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine.