Investigation of Synthetic Compounds in Drug Discovery by i-Series and LCMS-2020
Operational efficiency is an important issue to researchers involved in the organic synthesis stage of drug discovery typically conducted at pharmaceutical companies and universities.
Specifically, large numbers of drug candidates are synthesized daily in the drug discovery chemistry operations conducted throughout the pharmaceutical industry, and the synthesis and purity confirmation of these substances are primarily conducted by LC/MS. Typically, the LC/MS is treated as a shared instrument in an open-access environment, so rather than optimizing the analytical conditions for the various individual compounds, each type of compound is analyzed using a fixed set of analytical conditions. Further, the analyst conducting an analysis is often unaware of the type of compound that was analyzed by the previous analyst who used that instrument.
Reliable analysis in such an environment fundamentally requires an LC system that ensures low carryover performance. The i-Series integrated Ultra High Performance Liquid Chromatograph effectively suppresses carryover using specially treated liquid-contact surfaces, such as the needle seal and sampling needle, thereby providing excellent quantitative performance with high accuracy in a wide variety of analyses. Furthermore, the i-Series can be connected with the high-scan-speed LCMS-2020. The built-in UV / VIS detector or PDA detector along with connection to an LCMS permit very detailed qualitative and quantitative analysis.
Here, we introduce an example of analysis of eight pharmaceutical substances in a workflow that is typically used for pharmaceutical synthesis confirmation analysis.
Amid increasing calls for improved work efficiency and a more flexible working style, ideas of LC analysis are changing. The time has come for an HPLC that delivers rugged, reliable results with less frequent interaction by the analyst. The new, integrated i-Series LC system maintains the excellent performance of its predecessor while addressing the need for automation efficiency.
By incorporating the following innovative features into the LCMS-2020, the sharp peaks of ultra high-speed LC won't be missed:
・UFswitching (high-speed positive/negative ionization mode switching)
・UFscanning (high-speed scan measurements)
・UFsensitivity (superior sensitivity and repeatability thanks to newly developed ion optics)