SuperFlo Columns

Radial Flow Columns

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SuperFlo columns

Redefining the possibilities in preparative liquid chromatography

SuperFlo® Columns address the fundamental limitation of the axial column design by maintaining low pressure even as column size increases. The unique radial-flow design provides a significantly larger sample inlet area, which results in lower linear velocities, even at high total flow rates. SuperFlo columns are capable of extremely high flow rates and are ideally suited for high-throughput capture applications.

 

Features

    • True Linear Easy Scale-up
    • Validated Packing Method
    • High Flow Rates
    • Low Back Pressure
    • High Productivity
    • Higher Yields
    • Superior Distribution
    • Consistency in Performance

How SuperFlo Columns Work

  • The SuperFlo columns use a radial flow column (RFC) design consisting of two concentric porous cylinders that form the column bed. The space between the inner and outer frits is packed with the chromatographic resin. The eluent or sample enters through the column inlet at the top of the column. The top lid acts as a distribution head, directing the liquid into the space between the outer column body and the outer frit. The liquid is distributed over the entire height of the column and flows horizontally through the packed bed from the outer frit toward the inner core. It then passes into the space between the inner frit and the collector rod and exits through the column outlet at the bottom. The effective bed height is the distance between the inner and outer frits.
    The unique radial-flow design of the SuperFlo column provides a significantly larger surface area than traditional axial columns, maintaining optimal linear velocity and effective interaction between the sample and resin even at high flow rates.

  • structure

SuperFlo Column Sizes and Materials

  • SuperFlo columns are available in a range of standard sizes and can also be customized to meet specific process requirements. Standard acrylic columns are available in sizes from 50 mL to 1,500 mL with a 3.5 cm bed height. Standard stainless-steel SuperFlo columns range in size from 100 mL to 1,500 mL with a 3.5 cm bed height and are equipped with 10 μm stainless-steel frits. The columns offer excellent chemical compatibility across a pH range of 2 to 12.

  • Lineup
Purpose/Use Name Volume Bed Height/Depth Pressure Construction
Acrylic SS
Lab/R&D SuperFlo 50 50 mL 3.5 cm 50 psi Yes No
SuperFlo 100 100 mL 3.5 cm 50 psi Yes Yes
SuperFlo 250 250 mL 3.5 cm 50 psi Yes Yes
SuperFlo 500 500 mL 3.5 cm 50 psi Yes Yes
SuperFlo 1000 1,000 mL 3.5 cm 50 psi Yes Yes
SuperFlo 1500 1,500 mL 3.5 cm 50 psi Yes Yes
Pilot SuperFlo 2L 2 L 5 cm 50 psi Yes Yes
SuperFlo 3L 3 L 5 cm 50 psi Yes Yes
SuperFlo 5L 5 L 5 cm 50 psi Yes Yes
SuperFlo 7.5L 7.5 L 5 cm 50 psi Yes Yes
SuperFlo 10L 10 L 5 cm 50 psi Yes Yes
Process SuperFlo 10L 10 L 10 cm 50 psi Yes Yes
SuperFlo 20L 20 L 10 cm 50 psi Yes Yes
SuperFlo 30L 30 L 10 cm 50 psi Yes Yes
SuperFlo 40L 40 L 10 cm 50 psi Yes Yes
SuperFlo 50L 50 L 10 cm 50 psi Yes Yes
SuperFlo 60L 60 L 10 cm 50 psi Yes Yes
SuperFlo 70L 70 L 10 cm 50 psi No Yes
SuperFlo 80L 80 L 10 cm 50 psi No Yes
SuperFlo 90L 90 L 10 cm 50 psi No Yes
SuperFlo 100L 100 L 10 cm 50 psi No Yes
Industrial SuperFlo 150L 150 L 15 cm 50 psi No Yes
SuperFlo 350L 350 L 15 cm 50 psi No Yes

Case Study 1: Accelerating Plasma Separation with Radial Flow Columns

SuperFlo columns can significantly reduce processing time while lowering labor and operating costs. The data below were obtained from a plasma fractionation facility and compare the performance of a 20 L SuperFlo column with that of a 16 L axial-flow column. As shown in Table 1, the SuperFlo column achieved more than three times the flow rate, reducing processing time from three shifts to a single shift while maintaining product recovery and purity.

  • Productivity1
  • Productivity2

    Courtesy: Bayer Corp.

 

  • Pilot Scale

      Axial Flow Column SuperFlo Column
    Flow rate 25 L/hr 75-95 L/hr
    Production rate 350 L/3 shifts 350 L/shift
    Column size 16 L 20 L

  • Production Scale

      Axial Flow Column  SuperFlo Column
    Flow rate 200 L/hr 200 L/hr
    Production rate 1000 L/3 shifts 1000 L/shift
    Column size 160 L 60 L
    Cost of Packing $320,000 $120,000
    Initial Savings in Media Costs $200,000
    Scale-up using radial flow columns resulted in one-third the column size, one-third the processing time, and one-third the media cost.

Purification of an Intracellular Bacterial Enzyme

The example below demonstrates the purification of a recombinant protein. During scale-up from a 100 mL axial flow column to a 1,200 mL radial flow column, increasing the flow rate by threefold significantly reduced processing time. The shorter residence time also minimized on-column protein degradation that had occurred during longer processing, resulting in improved product recovery.

Isolation of recombinant proteins

Courtesy: Genencor

  • sample
  • sample
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