iMLayer AERO - Features

Automatic Sprayer for MALDI Imaging

Imaging with High Reproducibility

 
 
Humidity Control

Humidity Control

Humidity does not impact matrix deposition because the atmosphere within the spray chamber is replaced before pretreatment. Spraying can be performed under more stable conditions than with a hand spray.

Clog-Free Reagent Delivery

Clog-Free Reagent Delivery

If the matrix clogs the nozzle tip, the spray becomes unstable, which can lead to lower reproducibility. The rinsing mechanism allows for clog-free, stable spraying, which enables high reproducibility in MALDI analyses.

 

Both Sensitivity and Spatial Resolution Achieved

 
Humidity Control

Excellent Spray Performance

The standard protocol provides sensitivity on par with a hand spray along with improved resolution, homogeneity and reproducibility

 
 

Two-Step Vapor Deposition

To acquire high spatial resolution (5 to 10 μm) images and increase sensitivity, a two-step vapor deposition method can be implemented in combination with the iMLayer matrix vapor deposition system has been developed.* This unique experiment can only be implemented using Shimadzu sample preparation solutions.

* Patent No.: JP6153139 and JP6183779

 

Two-Step Vapor Deposition Allows for Very Fine Images with Minimal Blur

Hand Spray

Hand Spray

  • Matrix:9-AA
  • Volume used:200 μL
Two-Step Vapor Deposition

Two-Step Vapor Deposition

1 step:iMLayer(vapor deposition method)
 
  • Film thickness:1 μm
 
2 step:iMLayer AERO(spray method))
 
  • Solution delivery volume:120 μL/min
  • Stage speed:70 mm/sec
  • Laminating layers:4
 
 

Applicable to Pretreatment Other than Matrix Coatings

Derivatization Treatment

Derivatization Treatment

The system is also compatible with derivatization treatment (Girard's reagent T).
A single iMLayer AERO unit provides extensive MSI pretreatment coverage.
Using derivatization can improve the ionization efficiency of testosterone and other substances that are difficult to ionize.

 

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