Imaging Mass Microscope

With mass spectrometry imaging (MSI), suitable pretreatment is important. High quality MSI analysis results are obtained from a combination of the spray method and the vapor deposition method.

Work Flow

Automatic Sprayer
iMLayer AERO (Option)

The iMLayer AERO incorporates a sample stage that moves at a controlled rate while maintaining the same distance from the spray nozzle, enabling stable matrix spraying. Over multiple strokes, the sample becomes laminated with fine matrix crystals, enabling high sensitivity and high spatial resolution.

iMLayer AERO

Imaging with High Reproducibility

Humidity Control

Humidity does not impact matrix deposition because the atmosphere within the spray chamber is replaced before pretreatment. Spraying can be performed under more stable conditions than with a hand spray.

Humidity Control

Clog-Free Reagent Delivery

If the matrix clogs the nozzle tip, the spray becomes unstable, which can lead to lower reproducibility. The rinsing mechanism allows for clog-free stable spraying which enables high reproducibility in MALDI analyses.

Clog-Free Reagent Delivery

Matrix Vapor Deposition System
iMLayer (Option)

Applying the matrix by the vapor deposition method supports high resolution MALDI imaging.


Both Spatial Resolution and Sensitivity Thanks to the Two-Step Vapor Deposition Method

Two-Step Vapor Deposition

iA two-step vapor deposition method has been developed*, which  provides high spatial resolution (5 to 10 μm) and high sensitivity,  thanks to a combination of iMLayer (vapor deposition method) and  iMLayer AERO (spray method). This unique experiment can only be  implemented using Shimadzu sample preparation solutions.

* Patent No.: JP6153139 and JP6183779

Two-Step Vapor Deposition

Mass spectrometry imaging data analysis software

Automatically uncover important information from large amounts of data with IMAGEREVEAL MS.


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