LC/MS/MS MRM Library for Phospholipid Profiling
For LabSolutions™ LCMS
This MRM library includes two methods: one for phospholipid classification by comprehensive analysis of the main phospholipids in biological samples, and one for fatty acid composition determination created using analytical results obtained with the classification method. The library targets phospholipids containing C14 to C22 fatty acids, and includes MRM transitions for up to 867 components. This library enables performing phospholipid profiling by conducting an initial analysis with a phospholipid classification method. This is followed by creating a method for fatty acid composition determination based on the phospholipid peak detected in the first analysis, and subsequently using this method to perform a second analysis to determine fatty acid composition.
The phospholipids registered in the MRM library have fatty acid compositions with the carbon number and double bond combinations shown in the table below. The phospholipid targets of the library are phosphatidylcholines (PC) with a lyso-group, phosphatidylethanolamines (PE), phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylserines (PS), and sphingomyelins (SM).
MRM Transition Composition
Focusing on the characteristic phospholipid head group, the library includes a method for phospholipid classification and a method for fatty acid composition determination (for fatty acid compositions of the given combinations) that make use of these MRM transitions. The figure below shows each MRM transition required to identify PC (18:1/16:0). The phospholipid can be inferred by combining the analytical results obtained from these MRM transitions.
Step 1 Simultaneous Analysis Using the Phospholipid Classification Method
The phospholipid classification method is capable of comprehensive analysis of the main phospholipids in biological samples. It determines the phospholipid class based on the main characteristic head groups of phospholipids. Its analysis targets are phospholipids that include C14 to C22 fatty acids.
Step 2 Peak Identification
The phospholipid peak detected by the phospholipid classification method is identified. The structural information on the detected phospholipid at this point is the phospholipid class (PC, PE, PG, PI, PS, SM), and the total carbon number and number of double bonds in its constituent fatty acids. In the next step, the fatty acid composition determination method is created.
Step 3 MRM Event-Link Editor Editing the Fatty Acid Composition Determination Method
Software allows a fatty acid composition determination method to be edited from 867 MRM transitions for the phospholipid peak detected during first analysis.
Step 4 Simultaneous Analysis by Fatty Acid Composition Determination Method
Edited in MRM Event-Link Editor, the fatty acid composition determination method is used to perform a second analysis on the same sample. Phospholipid profiling can be performed based on the results of the first and second analysis.
Step 5 Data Analysis
Based on the analysis results, it was determined that the sample contains PC (16:0/20:1) and PC (18:0/18:1), as shown in the figures below.
Remarks and Precautions
LabSolutions LCMS Ver. 5.109 or later and LabSolutions Insight Ver. 3.8SP1 or later are required.
LabSolutions is a trademark of Shimadzu Corporation or its affiliated companies in Japan and/or other countries.
For Research Use Only. Not for use in diagnostic procedures.
News / Events
Handbook of Analysis Procedures for EPA method 533, EPA method 537.1 are now available.
The validity of the analysis performed by each laboratory needs to be confirmed by Quality Control (QC). This article section shows the general analytical procedure for fulfilling QC requirements. Note that the procedure could be modified depending on the situation in the lab and analysts.
Shimadzu has released the CLAM-2040, Fully Automated Sample Preparation Module for LCMS
The CLAM-2040 accompanies you on most of the analytical workflow and improves your overall throughput by drastically reducing the sample preparation time. Simply place the blood tubes in position and the CLAM-2040 performs the next steps automatically, from sample extraction, up to the LCMS analysis and data processing.
Per- and Polyfluoroalkyl Substances (PFAS)
Per-and polyfluoroalkyl substances (PFAS) are a group of persistent and harmful chemicals that can be commonly found in the environment globally. These anthropogenic chemicals can come from various sources such as manufacturing, industrial applications, food contact materials and consumer products, and eventually end up and accumulate in the water, soil and living organisms.
Interpretation of LC Chromatograms Facilitated by Mass Detection and the Mass-it™ Function
The LCMS-2050 mass spectrometer includes Mass-it functionality that can overlay compound mass (m/z) information, obtained with the mass spectrometer, onto UV chromatograms to support supplementing qualitative analysis results obtained using a UV detector.
Technical Background for the LCMS-2050, a Compact Mass Detector for LC
The LCMS-2050 single quadrupole mass spectrometer is highly compact in size, yet provides both high-speed and high-sensitivity analysis. Shimadzu’s technology has been condensed into an instrument miniaturized to the utmost.
Shimadzu has updated LC/MS/MS Method Package for Cell Culture Profiling Ver. 3 for LabSolutions LCMS
Version 3 of this method package adds metabolites characteristic of CHO cells, which are frequently used in the manufacture of monoclonal antibodies. Together with existing analytes, this Method Package enables the analysis of 144 compounds in under 20 minutes per sample.