LC/MS/MS Method Package for Aminoglycoside Antibiotics
For LabSolutions™ LCMS Software
- Complete method for fast quantitative screening of 13 aminoglycosides in meat, eggs and milk.
- Increase identification confidence with MRM Spectrum mode and LabSolutions Insight™ Screening.
- No ion-pairing reagent required.
- Includes single sample preparation protocol usable for meat, eggs and milk.
Complete Solution for Quantitation and Confirmation of Aminoglycosides in Meat, Eggs and Milk
Aminoglycosides are an antibiotic family widely used for the treatment of bacterial infections in cattle, sheep, pigs and poultry. Regulatory agencies have set maximum residue limits (MRL) for these compounds after veterinary use. Aminoglycosides are very polar compounds, and usually require the use of undesirable ion-pairing reagents with LC-MS/MS.
This Method Package is a complete solution for the simultaneous quantitative analysis of all regulated aminoglycosides by HILIC/MS/MS.
It includes:
- A sample preparation protocol using mixed-mode weak cation exchange from Biotage. This protocol produces high recoveries and low matrix effects, and is adapted to meat, milk or egg samples.
- A primary method for fast (< 5 min) quantitative analysis by HILIC/MS/MS with two MRMs per compound.
- A second method for confirmation of identity. Using the same mobile phases and column, the gradient is modified and 15 optimized MRMs are acquired per compound. This enables library searching and accurate identification on Insight Screening using MRM Spectrum Mode (library provided).
Targeted Aminoglycosides
- Amikacin
- Apramycin
- Dihydrostreptomycin
- Gentamicin (as Gentamicin C1, C1a and C2/C2a)
- Hygromycin B
- Kanamycin
- Neomycin B
- Netilmicin
- Paromomycin
- Sisomicin
- Spectinomycin
- Streptomycin
- Tobramycin
- Ribostamycin (internal standard)
Easy Workflow
Complete protocol includes step-by-step instructions for sample and calibration curve preparation.
Fast Quantitation and Easy Processing
Perform high-sensitivity analysis with excellent chromatography without resorting to ion-pairing reagents that foul the mass spectrometer.
LabSolutions Insight provides a fast and intuitive environment for data processing and review. User-configurable flags alert you what values are outside of QC limits, facilitating review by exception.
Increased Confidence in Identification using MRM Spectrum Mode
MRM Spectrum Mode acquires up to 15 MRMs per analyte, and each transition is optimized to a specific collision energy. Because MS duty cycle is not wasted on meaningless portions of the spectrum, information-rich spectra can be acquired in just milliseconds. The result is an unambiguous spectrum, even for trace-level components.
Note
1. LabSolutions LCMS Ver.5.91 or later is required.
2. LabSolutions Insight Library Screening Ver. 3.1 or later is required.
News / Events
-
GCMS-QP2050 Gas Chromatograph Mass Spectrometer Video has released
The business environments and needs involved in analysis work change on a continual basis. The next-generation GCMS-QP2050 gas chromatograph mass spectrometer, with its accumulation of impressive Shimadzu technology, will lead the way forward. New value is provided by hardware boasting astounding reliability and stability, and easy-to-operate software equipped with superior automated technology.
-
OAD-TOF system Interview with Dr.Hiroshi Tsugawa
The OAD-TOF system is a Q-TOF LCMS that realizes OAD (Oxygen Attachment Dissociation), Shimadzu's proprietary fragmentation technology. It allows the analysis of the position of carbon-carbon double bonds in lipids and other organic compounds. Combined with the LCMS -9050, which boasts world-class high mass accuracy and fast polarity switching, it enables the creation of innovative applications.
-
Metabolic Pathway Analysis Solutions
Metabolic pathways are the chain of chemical and enzymatic reactions that occur within a cell in living organisms to support their life.
-
AOAC INTERNATIONAL Posters are now available.
-
Shimadzu has released the OAD-TOF system
The OAD-TOF system is a Q-TOF LCMS that realizes OAD (Oxygen Attachment Dissociation), Shimadzu's proprietary fragmentation technology. It allows the analysis of the position of carbon-carbon double bonds in lipids and other organic compounds. Combined with the LCMS-9050, which boasts world-class high mass accuracy and fast polarity switching, it enables the creation of innovative applications.
-
Shimadzu has released the LC/MS/MS Method Package for Sugars and Sugar Nucle
The LC/MS/MS Method Package for Sugars and Sugar Nucleotides includes LC/MS/MS analytical methods with analytical conditions and MS parameter settings optimized for analyzing 25 different sugars, amino sugars, sugar acids and sugar alcohols, 9 different sugar nucleotides, related metabolites, and internal standards.