Principle of MALDI/TOFMS
What is MALDI?
MALDI is the abbreviation for "Matrix Assisted Laser Desorption/Ionization."
The sample for MALDI is uniformly mixed in a large quantity of matrix.
The matrix absorbs the ultraviolet light (nitrogen laser light, wavelength 337 nm) and converts it to heat energy. A small part of the matrix (down to 100 nm from the top outer surface of the Analyte in the diagram) heats rapidly (in several nano seconds) and is vaporized, together with the sample.
What is TOF MS?
TOF MS is the abbreviation for Time of Flight Mass Spectrometry.
Charged ions of various sizes are generated on the sample slide, as shown in the diagram. A potential difference V0 between the sample slide and ground attracts the ions in the direction shown in the diagram. The velocity of the attracted ions v is determined by the law of conservation of energy. As the potential difference V0 is constant with respect to all ions, ions with smaller m/z value (lighter ions) and more highly charged ions move faster through the drift space until they reach the detector. Consequently, the time of ion flight differs according to the mass-to-charge ratio (m/z) value of the ion. The method of mass spectrometry that exploits this phenomenon is called Time of Flight Mass Spectrometry.
MALDI-TOF Mass Spectrometer
The MALDI-7090 is targeted for proteomics and tissue imaging. It combines Shimadzu's extensive MALDI TOF-TOF mass spectrometry expertise with novel patented technology to provide ultimate performance in identification and structural characterization of biomolecules.
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Shimadzu is a leading driver in developing healthcare solutions with technology developments ranging from advanced imaging systems for use in medical diagnosis and treatment, engineering mass spectrometry-based solutions for exploring ways to discover disease biomarkers as well as building MS solutions for daily routine quantitation in biological fluids.
MALDI-TOF Mass Spectrometer Comparison
This page provides a quick overview of 7 models of MALDI/MALDI-TOF mass spectrometers. Choose the model that best suits your analysis needs from the compact digital ion trap MS for on-the-spot analysis, the benchtop MALDI-TOF MS for routine analysis to the research grade MALDI TOF/TOF MS for a diverse range of applications.
Shimadzu has released the MALDI-8030
The MALDI-8030 is the latest in a long line of MALDI-TOF products from Shimadzu. Instrument performance specifications is extended from those of the MALDI-8020 to cater for compounds best suited to analysis in negative ion mode. This dual-polarity, benchtop linear MALDI-TOF mass spectrometer delivers outstanding performance in a compact footprint, making it an ideal choice for today’s increasingly demanding laboratories.
Shimadzu has released the SialoCapper-ID Kit