Principle of MALDI/TOFMS
What is MALDI?
MALDI is the abbreviation for "Matrix Assisted Laser Desorption/Ionization."
The sample for MALDI is uniformly mixed in a large quantity of matrix.
The matrix absorbs the ultraviolet light (nitrogen laser light, wavelength 337 nm) and converts it to heat energy. A small part of the matrix (down to 100 nm from the top outer surface of the Analyte in the diagram) heats rapidly (in several nano seconds) and is vaporized, together with the sample.
What is TOF MS?
TOF MS is the abbreviation for Time of Flight Mass Spectrometry.
Charged ions of various sizes are generated on the sample slide, as shown in the diagram. A potential difference V0 between the sample slide and ground attracts the ions in the direction shown in the diagram. The velocity of the attracted ions v is determined by the law of conservation of energy. As the potential difference V0 is constant with respect to all ions, ions with smaller m/z value (lighter ions) and more highly charged ions move faster through the drift space until they reach the detector. Consequently, the time of ion flight differs according to the mass-to-charge ratio (m/z) value of the ion. The method of mass spectrometry that exploits this phenomenon is called Time of Flight Mass Spectrometry.
MALDI-TOF Mass Spectrometer
The MALDI-7090 is targeted for proteomics and tissue imaging. It combines Shimadzu's extensive MALDI TOF-TOF mass spectrometry expertise with novel patented technology to provide ultimate performance in identification and structural characterization of biomolecules.
News / Events
Shimadzu has released the FlexiFocus™
The FlexiFocus adaptor has been specifically designed to hold a FlexiFocus insert and position it optimally for analysis in the Shimadzu MALDI-8020 and MALDI-8030 benchtop MALDI-TOF instruments.
Latest issue of Shimadzu Journal, featuring Forensics / Toxicology, has come out.
This issue focuses on "Forensics / Toxicology", an area where science and technology are particularly important and needed. It starts with two customer interviews.
Life Science Biomarker Industry Page has been updated
Biomarkers are derived from living organisms and found in tissues, blood, and other bodily fluids. Biomarkers are indicators of biological changes in an organism and are used to diagnose disease, in prognosis, and to evaluate the efficacy and safety of pharmaceuticals. Biomarkers can be nucleic acids, proteins, peptides, lipids, and sugars, and are identified using various “omics” techniques.
Shimadzu has released the Benchtop MALDI-TOF Imaging Starter Kit
The Shimadzu imaging benchtop MALDI-TOF solution provides access for all to molecular imaging, through the introduction of a full imaging solution...
Dementia / Alzheimer‘s disease
Shimadzu is a leading driver in developing healthcare solutions with technology developments ranging from advanced imaging systems for use in medical diagnosis and treatment, engineering mass spectrometry-based solutions for exploring ways to discover disease biomarkers as well as building MS solutions for daily routine quantitation in biological fluids.
MALDI-TOF Mass Spectrometer Comparison
This page provides a quick overview of 7 models of MALDI/MALDI-TOF mass spectrometers. Choose the model that best suits your analysis needs from the compact digital ion trap MS for on-the-spot analysis, the benchtop MALDI-TOF MS for routine analysis to the research grade MALDI TOF/TOF MS for a diverse range of applications.