Benchtop MALDI-TOF Imaging Starter Kit - Applications

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Analysis of lipids in full rat brain with 30 µm and 50 µm spacing

Analysis of lipids in full rat brain with 30 µm and 50 µm spacing

MALDI images of full rat brain using FlexiVision-mini ITO glass slide. A, optical scan; B, positive ion mode image from MALDI-8020 using 50 µm spacing; C, negative ion mode image from MALDI-8030 using 30 µm spacing.

Sample B

 
Matrix DHB, sublimated with Shimadzu iMLayer device
Measurement region 67,024 pixels
Measurement time around 1.9 hours
Experiment details Laser repetition rate of 200 Hz and 20 shots per pixel

Sample C

 
Matrix 9-AA, sublimated with Shimadzu iMLayer device
Measurement region 275,210 pixels
Measurement time around 3.8 hours
Experiment details Laser repetition rate of 200 Hz and 10 shots per pixel

Large molecule imaging (protein/ on-tissue digestion) with 50 µm spacing

Large molecule imaging

MALDI images of protein and peptides in full rat brain using FlexiVision-mini ITO glass slide and MALDI-8020. A, MBP protein, (m/z 14124); B, MBP peptide (HGFLPR, m/z 726.39)

Sample A

 
Matrix Sinapinic acid, sprayed with Shimadzu iMLayer AERO device
Measurement region 68,836 pixels
Measurement time around 3.8 hours
Experiment details Laser repetition rate of 100 Hz and 20 shots per pixel

Sample B

 
Matrix CHCA, sprayed with Shimadzu iMLayer AERO device
Measurement region 67,482 pixels
Measurement time around 2.8 hours
Experiment details Laser repetition rate of 200 Hz and 30 shots per pixel