Evaluation of Kinetex Column Peak Capacity Using the Shimadzu Nexera UHPLC System

Introduction

Peak capacity is a commonly used metric of separation power that reflects the overall performance of the column and system. While peak capacity values can be increased with longer runtimes, today’s environment requires high resolution chromatography in a short amount of time. Both small particle and core-shell columns have shown the ability to deliver high resolution performance in a short period of time. A 24 second gradient runtime was evaluated with a sub 2 micron core shell particle column on the Nexera UHPLC system.

July 22, 2011 GMT