Comprehensive Cell Culture Profiling Using the LCMSTM-9030 Quadrupole TOF Mass Spectrometer

Introduction

In the production of useful substances by fermentation and the manufacturing of antibody drugs, the monitoring of culture media pH, dissolved gases, carbon sources (glucose), and nitrogen sources (glutamine) is implemented in order to optimize and manage the cultivation process. In addition to glucose and glutamine, the culture media components in cell cultures comprise vitamins and nucleic acid related compounds, as well as metabolites and other compounds secreted from cells. Accordingly, the comprehensive analysis of compounds contained in culture media is expected to provide useful information for discussing bioprocesses. Application News No. C106 introduced an example of the monitoring of changes in culture supernatant components in accordance with the hybridoma cultivation process, using a high-performance liquid chromatograph (HPLC) coupled with a triple quadrupole (TQ) mass spectrometer (MS) and a cell culture profiling method package. This Application News introduces an example of the monitoring of changes in culture supernatant components in accordance with the iPS cellular cultivation process using a HPLC coupled with a quadrupole time-of-flight (QTOF) MS. Changes in the components in a culture supernatant can be monitored comprehensively using a combination of targeted SIM and non-targeted full scan analysis via a LC-QTOF-MS.

March 22, 2019 GMT