Analysis of Oligosaccharides in Japanese Sake Using an Evaporative Light Scattering Detector

Introduction

An evaporative light scattering detector (ELSD) is an HPLC detector often called a "universal" detector because it can detect almost all non-volatile sample components, including those that do not absorb light. Although a differential refractive index detector (RID) can also be used for analysis of compounds with no chromophore, the ELSD removes any potential interference from the solvent peak that is eluted at the column void volume because detection occurs after volatilization and evaporation of the mobile phase. In addition, an ELSD detector has an advantage over an RID with its capability for analysis using gradient elution conditions. Here we introduce an example of analysis of oligosaccharides in Japanese sake using the Nexera-i integrated high-performance liquid chromatograph, which includes a built-in UV detector. The ELSD-LTII evaporative light scattering detector was connected directly to the Nexera-i through an A/D acquisition board.

November 26, 2014 GMT