Analysis of Choline and Acetylcholine in Rat Cerebrospinal Fluid Samples Using the Nexera UC-MS/MS System

Introduction

Choline, which is a structural element of cell membranes, and acetylcholine, which is known as a neurotransmitter, are both familiar compounds in the field of bioanalysis. Since acetylcholine is biosynthesized in the body from choline, it is possible to estimate the quality of internal activity by monitoring both of these compounds. This article focuses on the SFC analysis of these compounds in a rat cerebrospinal fluid sample by direct injection of the cerebrospinal fluid to the Nexera UC SFC system. Also introduced is automatic extraction and analysis of a cerebrospinal fluid sample impregnated into filter paper, in consideration of convenience and durability for storage and transport, using the Nexera UC online SFE-SFC-MS/MS system.

April 27, 2017 GMT