Analysis of Kakkonto by Nexera-e and SPD-M30A Photodiode Array Detector (Part 2)

Introduction

Kakkonto is a mixture of several traditional Chinese medicines. Type of compounds and their contained amount vary due to their origin. Here, we compared 2D contour plots of four kinds of kakkonto obtained by the Nexera-e comprehesive two- dimensional chromatograph detecting at UV 254 nm. Multiple peaks were observed on each chromatogram. When there are differences on comparison, a zoomed display and overlay of two 2D contour plots are available to conduct a differential analysis and compare them in detail. A 3D display is also available for checking the separation more visually. In this example, differences were observed between sample A and C (please see the black frames), which were not observed only by one-dimensional analysis. Detailed comparison is shown on the next page. Regarding sample pretreatment and analytical conditions, please refer to the previous application news No.L469.

September 5, 2014 GMT