A Study of Toxicity Evaluation Using the iMScope TRIO

Introduction

In drug discovery research, the analysis of the pharmacokinetics of candidate compounds provides important information not only in the elucidation of pharmacological mechanisms, but also from the viewpoint of toxicity assessment. In general, a method using autoradiography (ARG) and fluorescent dye is used, but with ARG the costs are high, and there have been concerns about the effects of using fluorescent agents as labeling agents on the pharmacokinetics. In recent years, the analytical technique of MS imaging has been attracting attention as a method that can detect data on the localization of candidate compounds without using a label. This method is expected to provide a breakthrough in drug discovery research as it can be used to analyze the localization of various substances without labeling, and to simultaneously analyze the unchanged drug and its metabolites using the same section. Here, we introduce an example of MS imaging analysis using the iMScope TRIO imaging mass microscope to compare the localization of the pathological findings with that of the amiodarone observed in lung tissue after administering amiodarone to rats.

July 3, 2017 GMT