Simple Evaluation of Cultured Cells: Evaluation of Differentiation State of iPS Cells Using Benchtop MALDI-TOF MS

Introduction

The growth condition and differentiation state (differentiated or undifferentiated) of cultured cells are important evaluation items in regenerative medicine and basic research. If these cell evaluations can be conducted in a simple manner by mass spectrometry, evaluation time and complicated work can be significantly reduced. Therefore, in order to examine the possibility of determining the state of cells by mass spectrometry, we conducted an automatic analysis of iPS cells and their culture media by AuraSolution using the Shimadzu MALDI-8020 benchtop linear MALDI-TOF mass spectrometer, and attempted a preliminary analysis by the statistical analysis software eMSTAT Solution. The results are reported in this article.

June 18, 2019 GMT