Quality Control Analysis of Synthetic Peptides Using the MALDI-8020 Benchtop Linear MALDI-TOF Mass Spectrometer and QC Reporter Software

Introduction

Within the manufacturing process of bio-therapeutics, quality control (QC) plays a fundamental role in guaranteeing the supply of a high quality product. Any changes in the product formulation or degradation can affect the therapeutic role, leading to a potential loss of activity or development of toxicity. Amongst the several analytical techniques that can be used to determine the quality of a synthetic bio-product, MALDI-TOF mass spectrometry is widely employed due to its rapid and simple operation, low running costs, sensitivity and ability to provide information on the molecular weight as well as the sequence and structure of a compound, its impurities/adducts and modification products. An example of a modification that can occur as result of degradation is the oxidation of methionine residues of peptides or proteins as this amino acid is highly susceptible to oxidation. Here, we provide a complete workflow solution for the high throughput, automated quality control analysis of synthetic products using the Shimadzu MALDI-8020 benchtop linear MALDI-TOF mass spectrometer and the QC Reporter software.

March 29, 2019 GMT