MS Imaging of Rat Liver Tissue Sections Using MALDI-7090

Introduction

In order to discuss the causes and disease progression, it is important to first clarify the diseased tissue, then determine the quantitative changes and distribution of the molecules present in that diseased tissue. Metabolic analysis by LC-MS or GC-MS is effective in ascertaining quantitative changes in metabolites and lipids, but due to the characteristics of the analytical methods, the information of molecular distribution in the diseased tissue is lost. For this reason, it is difficult to see the changes in molecular distribution associated with the disease progression. In recent study, MS imaging has been utilized as a method to solve this problem. In this article, we introduce an example of the analysis of the molecular dynamics of the disease progression in a non-alcoholic steatohepatitis (NASH) model rat using a combination of MS imaging and LC-MS or GC-MS analysis.

August 6, 2019 GMT