Simultaneous Analysis of Culture Supernatant of Mammalian Cells Using Triple Quadrupole LC/MS/MS

Introduction

Industrial fermentation for the production of biofuels or biopharmaceutics requires routine monitoring of medium conditions such as pH, dissolved gas, carbon source (glucose) and nitrogen source (glutamine) for optimization and control of the fermentation process. However, culture media also consist of various other biologically important compounds such as vitamins, nucleic acids and other primary metabolites, which would lead to more detailed understanding of the bioprocess if monitored altogether. To meet the demand for comprehensive analysis of medium component, we optimized the analytical conditions and developed this “Method Package for Cell Culture Profiling” that can monitor relative abundance of 95 compounds listed herein. Using this Method Package, we demonstrated the change in abundance of culture medium components associated with hybridoma growth over a period of 5 days.

July 8, 2016 GMT