Comprehensive Monitoring Method for Analyzing 158 Lipid Mediator Species Using the Ultra-Fast LCMS-8050

Introduction

Lipid mediator is a generic term for bioactive lipids, which are produced in the body and play a role in many biological functions. Simultaneous analysis of over 100 lipid mediator- related compounds has recently been developed by achievement of high speed and high sensitivity on a comprehensive LC/MS system. Comprehensive monitoring of the lipid mediators resulted in showing a causal relationship between lipid mediators and various disorders. A lot of isomers of lipid mediator species show the same molecular weight and MS/MS spectrum, therefore chromatographic separation is needed for identification. “LC/MS/MS Method Package for Lipid Mediator Ver. 2” was developed to simultaneously analyze 158 lipid mediator-related compounds by ultra-fast triple quadrupole mass spectrometry. This application data sheet presents a list of targets and an example for measuring lipid mediators in various biological tissues. Furthermore, 87 arachidonic acids and its metabolites, 18 EPA and its metabolites, 16 DHA and its metabolites, 11 ethanolamids, 23 metabolites of other fatty acids, Azelaoyl-PAF, PAF and Lyso-PAF are all included as registered compounds in the method in order to analyze a total of 158 components with optimized MRM transitions and retention times.

December 5, 2014 GMT