Analysis of Nivalenol, Deoxynivalenol, 3-Acetyldeoxynivalenol and 15-Acetyldeoxynivalenol Using Triple Quadrupole LC/MS/MS (LCMS-8050)

Introduction

Nivalenol and deoxynivalenol are mycotoxins which are produced by the fusarium fungi. A provisional reference value of 1.1 ppm was established in Japan for deoxynivalenol (Notification No. 0521001 issued by the Pharmaceutical and Food Safety Bureau, Japanese Ministry of Health, Labour and Welfare on May 21, 2002). The test methods specified for deoxynivalenol are HPLC for both qualitative and quantitative analysis, and LC/MS for verification testing (Notification No. 0717001 issued by the Dept. of Food Safety, Pharmaceutical and Food Safety Bureau, Japanese Ministry of Health, Labour and Welfare on July 17, 2003). This paper describes an LC-MS/MS method for high- sensitivity simultaneous analysis of the four compounds, nivalenol, deoxynivalenol and the deoxynivalenol metabolytes, 3-acetyl-deoxynivalenol and 15-acetyl- deoxynivalenol.

April 3, 2015 GMT