Difference in Quantifiable Concentration Ranges of UV-Vis Spectrophotometer and Fluorescence Spectrophotometer

Introduction

Both the UV-Vis (ultraviolet-visible) spectrophotometer and the fluorescence spectrophotometer are used in quantitative evaluations. Quantitative analysis by the UV-Vis spectrophotometer measures absorbance, which is proportional to concentration, based on the Lambert-Beer law. The fluorescence spectrophotometer uses fluorescence intensity and enables quantitative evaluation of low concentrations because the fluorescence intensity is proportional to concentration. In this experiment, we measured a rhodamine B solution with both a UV-Vis spectrophotometer and a fluorescence spectrophotometer. Rhodamine B is a fluorescent substance which is used in dyes for textiles and leather. This article introduces, based on the measurement results, the lower limits of quantitation and detection and the linearity of the calibration curves of the two instruments were compared.

August 5, 2020 GMT