High Speed Analysis of Monosaccharides and Disaccharides in Soft Drinks by ELSD-LT III

Introduction

Saccharides show very narrow UV absorption wavelength range, from 190 nm to 195 nm because hydroxyl group is the only major functional group that show UV absorption in the structure. Therefore, a refractive detector (RID) is commonly used for this analysis. However, gradient elution cannot be used with RID because the baseline drifting derived from the change of mobile phase composition during gradient elution is practically unacceptable. So, RID is not suitable for a simultaneous separation of compounds that show widely different retention behaviors due to expected long analysis time without gradient elution. Evaporative light scattering detector (ELSD) is one of universal detector that detects the scattering light from the target compounds after nebulizing and evaporating the mobile phase. ELSD provides reduced analysis time and simultaneous separation of compounds that show widely different retention due to applicability to gradient elution. Monosaccharides and disaccharides are mainly analyzed by anion-exchange chromatography or ligand exchange chromatography. However it takes a long time to analyze. So, in this article, monosaccharides and disaccharides were separated in short time by hydrophilic interaction chromatography (HILIC) and were detected by ELSD .“Wide function”, a new feature of ELSD-LT III used in this article, automatically optimizes a parameter that is related to sensitivity and a single method file can be used for data acquisition regardless of sample concentration, from low to high. Here, high speed analysis of monosaccharides and disaccharide in soft drinks were carried out by ELSD-LT.

September 1, 2020 GMT