Analysis of Amino Acids by KBr Tablet Method

Introduction

In the transmission method, light is irradiated on a measurement sample, and the light that passes through the sample is detected. However, the adequate technique (accessory device) differs depending on the sample shape. Among techniques used with the transmission method, the KBr tablet method is mainly used to measure solids and powders. The KBr tablet method is a necessary and indispensable procedure which has been standardized not only in the Japanese Pharmacopoeia (JP), but also in the United States Pharmacopeia (USP), standards of the American Society for Testing and Materials (ASTM). Special care is necessary in order to obtain a satisfactory spectrum by the KBr tablet method, including securing a uniform powder size and maintaining a constant pressure during tablet forming (pelletizing). In particular, the effect of moisture requires special attention. In this experiment, we conducted an analysis of amino acids, which easily absorb moisture, by the KBr tablet method. This introduction also includes an example of failure.

March 23, 2020 GMT