Fast Analysis of Organic and Amino Acids in Café Late by Solid Phase-Derivatization method

User Benefits

- 10 mins sample extraction with SP-Deriv. method followed by 23 mins MRM run (Smart Metabolites Database™) - 5 min analysis time with TraverseMS - Selective removal of carbohydrates from sugar rich food samples

Introduction

Food products with a high concentration of sugars are known to pose extraction issues both during a freeze drying (e.g. loss of sample by explosive boiling) as well as in the subsequent derivatization steps (e.g. derivatization reagent quenching). As a solution to these issues, SP-Deriv. method using Presh-SPE (Wakayama, Japan, AiSTI Co., Ltd) was employed to selectively remove carbohydrates from sugar rich samples before derivatization steps. In this article, results from the conventional overnight method (i.e. Bligh and Dyer extraction, drying and derivatization) and the SP-Deriv. method were compared.

December 10, 2020 GMT