Evaluation of Differentiated/Undifferentiated State of iPS Cells Using DPiMS™-2020 and eMSTAT Solution™ (B108)

Introduction

The differentiated/undifferentiated state of pluripotent stem cells is a critical evaluation item in research and development and quality control in the field of regenerative medicine. Because the conventional method for evaluation of the differentiated/undifferentiated state required fractionation and pretreatment of some cultured cells, quick analysis was difficult, and it was necessary to use part of the valuable cultured cells. If data on the differentiated/undifferentiated state could be obtained from a small sample by simple pretreatment, quick evaluation with minimal loss of cells would be possible. This article introduces an example of quick and simple measurement of the components contained in iPS cell culture medium with a DPiMS-2020 mass spectrometer using the probe electro spray ionization method, which is a new ionization method. This article also introduces an example in which grouping of the culture media of differentiated/undifferentiated cells was possible by using eMSTAT Solution statistical analysis software.

February 4, 2020 GMT