Development of Profiling Method for Major Lipids in Blood by Triple Quadrupole LC/MS/MS

User Benefits

- Profiling analysis of the major lipids in blood is possible. - The LCMS-8060 can acquire high quality data, even in positive/negative simultaneous analysis (polarity switching time: 5 msec). - Fatty acid composition analysis of the major lipids is possible using small amount of human blood.

Introduction

The major lipids in blood are phospholipids (PL), triacylglycerols (TG), and cholesteryl esters (CE). The fine structures of these major lipids have attracted attention in connection with various types of diseases and in research on pathophysiology. The authors developed a phospholipid profiling method based on multi-reaction monitoring (MRM) using the LCMS-8060, as reported in Application News No. C137. This article introduces the development of a profiling method which widens the range of targets to the major lipids in blood, including not only the phospholipids, but also CE, TG, free fatty acids, and cholesterol. Approximately 360 MRM transitions were prepared in this method. It was possible to identify approximately 100 types of lipids in blood, including 51 PLs, 26 TGs, 11 CEs, CE hydroperoxides, free fatty acids, and free cholesterol. It was also possible to apply this method to blood plasma and blood serum, and the compositions of the fatty acids of various classes of lipids were clarified.

March 4, 2021 GMT