SialoCapper-ID Kit : A Novel Derivatization Tool for Glycan Analysis by Mass Spectrometry

User Benefits

- Sialic acid linkage types scan be discriminated using only MS analysis without comparison to standard glycan analysis results, which can contribute to laboratory labor-savings. - Sialoglycan sensitivity is improved, enabling analysis of even trace quantities without wasting precious samples. - The kit is highly compatible with conventional glycan analysis methods and can be flexibly incorporated into existing experimental procedures.

Introduction

Known as the “third chain of life,” glycans exist in various forms in the body. Glycosylation of proteins is closely related to the activities of hormones and biopharmaceuticals, as well as viral infections and diseases such as cancer. Consequently analyzing the structure of glycans is essential for understanding biological phenomena and the beneficial effects of pharmaceuticals. Sialic acids (SA) refer collectively to the family of acidic mono- saccharides that are usually the outermost component of a glycan. There are many biological phenomena related to sialic acids. For example, sialic acids play a central role in the influenza virus infection process. HPLC analysis with fluorescent labeling previously was the gold standard for glycan analysis, but in recent years, highly sensitive and high-throughput mass spectrometry (MS) has been widely used. However, the analysis of acidic glycans with sialic acid residues is more difficult than that of neutral glycans. This article describes a novel derivatization method and a corre- sponding new SialoCapper-ID reagent kit for facilitating the MS analysis of glycans (sialoglycans) that comprise sialic acids.

April 28, 2021 GMT