Development of a Fast Eicosanoid Profiling Method Using Plasma Microsamples

User Benefits

- Eicosanoids and related fatty acids in plasma can be quantified in 10 minutes. - Nexera^TM and the LCMS-8060 ultra fast triple quadrupole mass spectrometer provides high throughput analysis. - The MSW² microsampling device enables the accurate collection of trace quantity plasma samples.

Introduction

Eicosanoids and metabolites of omega-3 fatty acids are important targets when investigating pathophysiological functions and identifying disease biomarkers. The authors have developed a method based on multiple reaction monitoring (MRM) for the quantitative identification of over 100 fatty acid metabolites in disease model mice serum (reported in ASMS 2019). To increase speed and sensitivity, the number of MRMs was narrowed down to the detection targets and internal standards. As a result, the authors have created a 10-minute LC-MS/MS method consisting of 200 MRMs for the monitoring of 114 target eicosanoids and related fatty acid metabolites. This Application News describes an analysis using this method in which eicosanoids and related metabolites were detected in 5.6 μL of healthy human plasma collected using a microsampling device (MSW).

April 28, 2021 GMT