An Easy and Fast Approach for Monoclonal Antibody N-linked Glycan Analysis from Sample Preparation to Data Analysis

User Benefits

- A simple and straightforward sample preparation workflow for N-glycan analysis - Less time-consuming for sample preparation - Improved peak resolution for better quantification

Introduction

Therapeutic monoclonal antibodies (mAbs) and their derivatives are emerging as one of the fastest-growing categories of biologic drugs with a wide range of applications. N-linked glycosylation plays a critical role in many biological processes, and may affect the therapeutics’ bioactivity, stability and immunogenicity. To maintain consistent glycosylation profiles of mAbs during manufacturing, effective glycan characterization are required, usually relying on techniques such as fluorescence-tagging coupled with HPLC analysis. The traditional method of N-glycan analysis is timeconsuming, and involves multiple steps, starting with releasing of glycan, followed by glycan purification, labeling with a fluorescence tag (e.g., 2- aminobenzamide, 2-AB), and finally cleanup of labeled glycans prior to LC analysis. This study demonstrates a simplified workflow using the S-Bio EZGlycoTM mAb-N kit, potentially reducing sample preparation time for Nglycan characterization. The solution incorporates the Shimadzu Nexera-i MT system and highly sensitive fluorescence detector (RF-20A).

May 13, 2021 GMT