A Sample Prep-Free Analysis of Saccharides Mixtures with “Smart IS+” and “SMCI+” - Authentication and Adulteration Studies -

User Benefits

- “Smart IS+” and “SMCI+” setups enable direct and quick qualitative GC/MS analysis of saccharides without the need for tedious derivatization steps. - “SMCI+” setup delivers convenience and safety to carry out positive chemical ionization and requires only common laboratory solvents as the reagent gas; Alternate between electron ionization and positive chemical ionization modes with “Smart IS+” - “Smart IS+” and “SMCI+” enable quick preliminary authentication and adulteration studies of saccharides due to their unique temperature elution profiles

Introduction

Saccharides have long been established as crucial energy storage materials, structural components, and primary metabolites that play major roles in physiological development. Saccharides can be divided into four groups, mono-, di-, oligoand polysaccharides. Mono- and disaccharides are the basic building blocks for the larger saccharides. Due to the high degree of functionality in the molecules, it is often necessary to protect all hydroxyl groups before analysis with the gas chromatography/mass spectrometry (GC/MS) technique. The usage of a direct probe as a sample inlet in GC/MS provides an alternative technique that eliminates the need for tedious sample preparation. This benefit paves the way for a quick and direct method to obtain preliminary results in product authentication and adulteration investigations. This article demonstrates the application of a direct probe in conjunction with a Smart EI/CI ion source (Smart IS) or solvent mediated chemical ionization (SMCI) unit for the analysis of saccharides. The mass spectra of saccharides generated by Smart IS and SMCI units will be evaluated, followed by examples of their usage for preliminary authentication and adulteration studies.

May 27, 2021 GMT