A Sample Prep-Free Analysis of Triglycerides and Fatty Acids with “Smart IS+” and “SMCI+” - Authentication Studies -

User Benefits

- “Smart IS+” and “SMCI+” enable derivatization-free procedures for qualitative GC/MS analysis of fatty acids and triglycerides - Easily switch between both electron ionization and positive chemical ionization modes with “Smart IS+” setup and “SMCI+”with methanol reagent gas produces characteristic mass spectra for saturated fatty acids - “Smart IS+” and “SMCI+” support quick preliminary authentication study of vegetable oil, e.g., coconut oil and sunflower oil

Introduction

Triglycerides are the primary constituents of body fat in animals as well as vegetable fat. Triglycerides are tri-esters that constitute glycerol and three fatty acids. The analysis of triglycerides and fatty acids is crucial to refining the current physiological understanding of animals and plants. The direct analysis of free fatty acids (FFAs) and triglycerides (TGs) with gas chromatography/mass spectrometry (GC/MS) technique requires specialized columns. Otherwise, multi-step transesterification or derivatization procedures are necessary to enable GC/MS analysis. The usage of a direct probe as a sample inlet in GC/MS provides an alternative technique that eliminates the need for tedious sample preparation. This benefit paves the way for a quick and direct method to achieve preliminary detection and identification of FFAs and TGs. This article demonstrates the application of a direct probe in conjunction with a Smart EI/CI ion source (Smart IS) or solvent mediated chemical ionization (SMCI) unit for the analysis of FFAs and TGs. The mass spectra of FFAs and TGs generated by Smart IS and SMCI units will be evaluated, followed by examples on their usage for preliminary authentication study of vegetable oils.

May 27, 2021 GMT