Simultaneous Determination of 15 Saccharides Without Interference by Salt in Seasonings

User Benefits

- The gradient elution method enables highly sensitive analysis of 15 saccharides from monosaccharides to oligosaccharides. - It is possible to separate glucose and galactose, which are known to coelute. - Quantitation of sugars that exist in small amounts is possible by desalination, even when analyzing foods with large salt contents.

Introduction

The relationship of sugars to factors that cause diabetes, obesity, allergies, and dementia has become clear. Although limiting sugar intake is effective for prevention and treatment of these conditions, seasonings tend to be overlooked when considering sugar limits, even though some seasonings actually contain large amounts of sugar. In addition to sugar, some seasonings also contain a large amount of salt. In quantitative analysis of samples with high salt contents, salt frequently interferes with the analysis of sugars when using detectors with limited selectivity, such as a differential refractive index detector (RID) or an evaporative light scattering detector (ELSD). In this article, quantitation accuracy was improved by desalination of the real samples in the sample preparation process. This article introduces analyses in which 15 saccharide compounds were separated by hydrophilic interaction liquid chromatography (HILIC) and detected with an ELSD-LT III detector. In addition, analyses of the saccharides in real samples of six types of commercially-available seasonings are also introduced.

May 27, 2021 GMT