Automated Analysis of Thirty-seven D/L-amino Acids using Liquid Chromatography with Fluorescence Detection and Its Application to Liquor Samples

User Benefits

- Separation and determination of thirty-seven D/L-amino acids in a short time with simple operation are possible. - The automatic operation reduces the labor and more than an hour of the working time per 20 samples consecutive analyses. - The elapsed time of the derivatization reaction is kept constant, resulting in good reproducibility of determination.

Introduction

Amino acids have D/L enantiomers that have asymmetric centers in their molecular structures except for glycine. In contrast to L-amino acids, there has been limited studies of Damino acids. The role of D-amino acids in the palatability, preservability, and the aroma of food and foodstuff has been largely unknown until recently. However, fermented foods and biological samples are known to contain small amounts of Damino acids in addition to large amounts of L-amino acids. Therefore, the demand for D/L separation of amino acids is increasing. This article introduces the analyses of fluorescent diastereomers of the proteinogenic D/L-amino acids using derivatizing reagents with chiral structures. In addition, it also introduces the automated analysis including derivatization.

June 29, 2021 GMT