Determination of Protein Secondary Structures of Monoclonal Antibody using FTIR Spectroscopy

User Benefits

- Micro liquid sampling with MicromATRTM ATR accessory - Increased sensitivity with IRTracer-100 and 9 reflection MicromATR ATR accessory

Introduction

Monoclonal antibodies (mAbs) are a major class of biopharmaceuticals due to its wide application in medicine and biological science. The antibody’s biological activity can be attributed to its unique structural conformation. Protein higher order structure (HOS) includes the secondary, tertiary and quaternary structures of protein, which comprise the three dimensional structures of protein. Analysis of the HOS of mAbs is essential to ensure the quality and efficacy of the protein therapeutics product. Some analytical methods that have been used to characterize protein HOS include UV circular dichroism, nuclear magnetic resonance (NMR) and fourier transform infrared (FTIR) spectroscopy. FTIR spectroscopy is suitable to determine the relative amount of different secondary structure of proteins. This information can be obtained from the amide band I of protein in the IR spectrum, ranging from 1600 cm-1 to 1700 cm-1 . Mathematical procedures such as band curve-fitting and second derivatives can be applied to resolve the overlapping amide I band components and quantify the secondary structure of proteins. In this application news, the secondary structure of mAbs is examined by using FTIR spectroscopy and band curve-fitting data analysis.

July 6, 2021 GMT