Analysis of Water-Soluble Vitamins in Foods by LCMS-8060NX Triple Quadrupole Mass Spectrometer

User Benefits

� - Simultaneous quantitative analysis of water-soluble vitamins with largely different contents is possible. �- Because the IonFocus^TM unit introduces ions into the MS with high efficiency, both excellent system robustness and high sensitivity analysis are achieved when moving the ion spray away from the ion inlet port to mitigate contamination. �- The analysis results show a high recovery rate even in analysis of complex food matrices such as modified milk powder (powdered infant formula) and milk raw material.

Introduction

Vitamins are classified as water-soluble vitamins or fat-soluble vitamins depending on their nature. Although the general analysis techniques for water-soluble vitamins include microbiological methods (bioassay), fluorescence methods, and the HPLC method, with the exception of HPLC, only one vitamin can be measured by those analysis methods. Thus, the time and labor required with those methods is a drawback. The HPLC method enables simultaneous measurement of multiple vitamins, but depending on the vitamin, the content in foods may be trace amount, and in some cases, this is an obstacle to analysis in complex matrices. With the LC/MS/MS method, simultaneous quantitative analysis of vitamins with higher efficiency is possible because vitamins can be analysed with selectivity and high sensitivity even in complex matrices. However, contamination of the MS is a problem when measuring complex matrices like those of foods. As one solution to this problem, the effects of contaminants can be minimized by separating the ionization unit from the MS ion introduction unit. The IonFocus Unit of the LCMS-8060NX efficiently introduces only ions into the MS and enables high sensitivity analysis while minimizing contamination of the MS unit because the ionization unit is separated from the MS ion introduction unit. In this article, we report the development of an analysis method for four water-soluble vitamins and an example of simultaneous analyses of the vitamins in food products using the LCMS- 8060NX.

July 16, 2021 GMT