Quantification of glycated hemoglobin as long-term control of diabetes

User Benefits

- Promising method for a routine control in diabetes treatment. - Easy sample preparation. - High-speed method without chromatography

Introduction

Matrix-Assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry (MALDI-TOF MS) facilitates a simple and quick analysis to obtain molecular weight information on diverse types of samples from small to macromolecules. Thus, there is a wide application range for MALDI-TOF MS instruments in R&D and quality control of synthetics and biological molecules. MALDI-TOF MS is widely spread in high-throughput routine e. g. in microbial ID in clinics. As many MALDI applications go without time consuming chromatography step, MALDI enables high-speed analysis. Long-term control of the glycemic state of hemoglobin is the most important and reference tool for the management of diabetes. The Dutch diabetic association recommends monitoring the level of glycated hemoglobin (HbA1c) two to four times a year, depending on the type of diabetes. Several procedures and numerous commercial instruments, based mainly on chromatographic separation methods, are currently available for the determination of HbA1c in blood samples. MALDI can help to reduce analysis time and therefore increase the number of samples that can be analyzed per day and instrument.

September 21, 2021 GMT