High-Speed and Simultaneous Analysis of Active Ingredients CPC and GK2 in Mouthwash

User Benefits

- Within 4 minutes, simultaneous analysis of two types of active ingredients is possible. - Basic compounds such as quaternary ammonium salts can be analyzed with good repeatability without peak tailing. - No ion-pairing reagents including perchloric salts (perchlorates) are used in the mobile phase.

Introduction

Commercial oral care products (mouthwashes) contain multiple active ingredients, which are generally analyzed by HPLC. When the quaternary ammonium salt CPC (cetylpyridinium chloride) is analyzed with a C18 column, it is known that the CPC will interact with residual silanol groups remaining on the surface of the silica gel packing material, causing adsorption and peak tailing. In recent years, columns which employ end-capping technology to mask residual silanol groups have appeared on the market. However, depending on target compounds, the effect in suppressing residual silanol groups may be slight, and in some cases peak tailing may occur. In such cases, adsorption of target compounds can be suppressed by adding an ion-pairing reagent or perchlorate to an acidic mobile phase. However, using a Shim- pack Arata^TM C18 column, interaction with residual silanol groups can be suppressed, and satisfactory peak shapes can be expected without addition of an ion-pairing reagent. This article introduces a high-speed and simultaneous analysis of CPC and GK2 (dipotassium glycyrrhizinate), which are active ingredients used in mouthwashes.

November 2, 2021 GMT