Development of an Analytical Method for Blood Triglycerides Using Triple Quadrupole Mass Spectrometer

User Benefits

- The method allows the measurement of 47 triglycerides of different molecular weights in 11 minutes. - Enables estimation of the combination of fatty acids in triglycerides. - Useful for biomarker research by high-throughput screening.

Introduction

Triglycerides (TGs) are important energy storage molecules in animals. They play an important role in transporting energy in blood, but excess triglycerides are thought to promote atherosclerosis or other circulatory diseases. Conventional blood tests estimate the total amount of triglycerides but do not provide quantitative information about the fatty acids bound to the various triglycerides. This article describes an LC/MS/MS method developed for the analysis of triglycerides in blood. The method uses 195 multiple reaction monitoring (MRM) transitions to allow the measurement of 47 triglycerides with different molecular weights in 11 minutes (130 analyses/day). Using this method, we analyzed two commercially available human plasma samples and one human serum sample. The slight differences between samples were captured by multivariate analysis. This method is available as the ”LC/MS/MS MRM Library for Triglycerides. ”

November 2, 2021 GMT