Measuring Primary Metabolites in Human Plasma Using an LC/MS/MS System with Fully Automated Sample Preparation Module

User Benefits

- Capable of comprehensive quantitative analysis of primary metabolites in plasma - Performs time-consuming sample preparation automatically. - Eliminates the need for manual sample preparation and reduces variability in quantitative results due to manual operations.

Introduction

Metabolomics analysis, a technique for comprehensive analysis of large numbers of metabolites, is used in a wide range of fields and applications, including functional analysis of food, improving fermentation productivity, and elucidating physiological and pathological mechanisms. Liquid chromatograph mass spectrometers and other mass spectrometer systems are used to perform metabolomics analysis. In recent years, metabolomics analysis is increasingly being used in clinical research, such as to search for disease markers and markers that predict the efficacy and toxicity of drugs. However, sample preparation for metabolomics analysis and the operation of mass spectrometers is much more complex than for general laboratory testing. As a result, there is a risk of procedure errors and variability due to operator differences. In addition, operator workload increases as the number of samples increases, and sample preparation can become a bottleneck in the analytical workflow when analyzing a large number of samples. A normal sample preparation protocol for primary metabolite analysis performed on an LC/MS/MS system with fully automated sample preparation module involves steps such as adding organic solvent for deproteination, removing solid components by centrifugation, and recovering supernatant. This article introduces an example of using an LC/MS/MS system with fully automated sample preparation module, comprised of a CLAM-2030 fully automated sample preparation module and LCMS-8060 liquid chromatograph mass spectrometer, to perform an analysis and resolve the issue of sample preparation encountered when metabolomics analysis is applied to clinical research.

January 6, 2022 GMT