Screening and Semi-Quantitation by LC/MS/MS in Whole Blood Using Rapid Tox Screening

User Benefits

- A simple extraction procedure using a commercially available Micro Volume QuEChERS kit. - LabSolutions Insight Library Screening makes it possible to confirm the result of spectrum library search. - A quick way to screen for 231 forensic compounds and confirm the semi-quantitative results without standard samples.

Introduction

Forensic toxicology is an important tool in the field of forensic medicine and death investigation. Since investigations are often time sensitive, a rapid analysis system to provide toxicologists and medical examiners with timely information would be beneficial. Recent developments in the robustness, sensitivity, and affordability of LC/MS/MS systems have made their presence in forensic laboratories a necessity. A quick and robust screening system with the ability to semi-quantitate whole blood samples called LC/MS/MS Rapid Toxicology Screening System has been developed. This system contains the method parameters for the identification and semi-quantitation of 231 forensically relevant compounds. The increased sensitivity of these systems allows for analysis of only 100 microliters of whole blood using a Micro Volume QuEChERS kit. This article details the sample pretreatment and results from analysis of an actual autopsy whole blood sample using the LC/MS/MS Rapid Toxicology Screening System Ver.3.01.

January 6, 2022 GMT