Method for the determination of 243 Residual Pesticides in Red Chili Powder using LCMS-8050 and GCMS-TQ8040 NX

User Benefits

- The method involves study of LOQ on both GC-MS/MS and LC-MS/MS, based on validation parameters like linearity, recovery, repeatability and within-laboratory reproducibility. - A modified QuEChERS extraction procedure has been employed for quantifying pesticides at trace levels in complex matrix like Red chili powder using Ultra-fast technologies of LCMS-8050 and GCMS-TQ8040 NX. - LCMS Method Package for Residual Pesticide Ver.3 and GCMS Smart Pesticides Database^TM Ver.2 from Shimadzu Corporation enables ease of optimizing instrumental method.

Introduction

Red chili powder is the dried, pulverized fruit of varieties of red peppers. It is used as a spice (or spice blend) to add pungency and flavor to culinary dishes. It is widely used in many different cuisines throughout the world. Owing to its high demand worldwide, the intensive use of agrochemicals during its cultivation has given rise to concerns over consumer’s exposure to pesticide residues and associated health risks. Hence, it is important for chili powder producers and regulators to ensure that presence of residual pesticides meet compliance criteria. Therefore, Maximum Residual Limits (MRL) are listed by various international regulatory bodies for wide variety of pesticides. Thus, increasing the importance of analytical method for determination of a range of pesticides present in red chili powder. This study reports a validated method for the determination of 243 pesticides in red chili powder by using LCMS-8050 and GCMS-TQ8040 NX. The multi-residue extraction was performed with modified QuEChERS[1] method for simultaneous quantification of 146 pesticides by LC-MS/MS and 122 pesticides by GC-MS/MS. Out of these, 25 pesticides were common and analyzed by both the techniques.

January 14, 2022 GMT