Achieving Improved Sensitivity and Reliable Analytical Performances in Nucleotides Analysis

User Benefits

- The inert UHPLC system suppresses the interaction of compounds containing phosphate group(s) with internal surfaces. - The combination of Nexera XS inert with a metal-free column improves peak shapes leading to better sensitivity and quantitative performance. - The Nexera XS inert improves the overall performances by eliminating the need for conditioning procedures before the analysis.

Introduction

Stainless steel is commonly used in HPLC due to its pressure proof and corrosion resistant. However, it can interact with compounds containing phosphate group(s) by metallic affinity. This is a factor that negatively affecting the shape and intensity of the peak. In order to suppress metal adsorption, cleaning flow path with phosphoric acid, addition of chelating agents to mobile phase, or repeated injections of the target compounds are often performed. Nevertheless, it is not easy to obtain highly reproducible results. This article introduces the use of the Nexera XS inert ultra-high performance liquid chromatograph, which utilizes a metal-free flow path, and a metal-free column for the accurate and reproducible analysis of nucleotides.

February 2, 2022 GMT