Simultaneous Analysis of Constituent Sugars and Glucuronic Acid in TEMPO-Oxidized Cellulose Nanofiber

User Benefits

- This method enables simultaneous analysis of the constituent sugars and glucuronic acid in TEMPO-oxidized cellulose nanofiber. - High sensitivity and selectivity analysis of saccharides is possible by using the post-column fluorescence derivatization method.

Introduction

Cellulose nanofiber (CNF) is produced by mechanically treating wood pulp (fiber diameter: 20 to 30 μm, fiber length: 0.5 to 3mm). However, because strong hydrogen bonds form between the CNF microfibrils in wood pulp, various problems arise if only mechanical treatment is used, as nanoization to the smallest crystal size (fiber diameter: 3 to 4 nm) is insufficient, damage to the CNF is excessive, and a large amount of energy is required in nanoization. A group led by Special Research Professor Akira Isogai of the University of Tokyo has reported that nanofibers can be obtained by TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl radical) mediated oxidation of cellulose and is promoting mass production by this approach. The Application News 01-00023-EN introduced an example of constituent sugar analysis in various CNF samples using a Shimadzu Nexera reducing sugar analysis system. This article introduces a simultaneous analysis of the constituent sugars and glucuronic acid in TEMPO-oxidized cellulose nanofiber.

April 5, 2022 GMT