Optimal matrix application methods for MS imaging

User Benefits

- The iMLayer enables users of any experience level to apply matrices easily and with high repeatability. - With the vapor deposition method, the matrix can be applied evenly and with very small crystal size. - The 2-step method involving spraying after vapor deposition allows MS imaging with high sensitivity and high spatial resolution.

Introduction

The technique of MS imaging, initially common only in drug discovery and metabolomics research, has become more widespread in a variety of fields over recent years. In the case of MS imaging using MALDI as the ionization method, it is particularly important not only to select the most appropriate matrix for detection of the target compound, but to consider the optimal matrix application method. Spraying of the matrix provides effective extraction from the biological tissue. However, this method has shortcomings in that the compounds may spread outside the tissue sample and the matrix crystals are large and non-uniform. On the other hand, the vapor deposition method of matrix application results in very small and uniform crystals, but does not provide effective extraction. In light of this, we have developed a 2-step method (Patent No: 6153139) which combines spraying and vapor deposition, as well as a recrystallization technique that can be used on the vapor-deposited matrix. In this document, we report the differences observed in matrix crystal size and shape depending on the type of matrix and the application method used, and introduce examples showing the benefits of the 2-step method for MS imaging.

April 5, 2022 GMT