Confirmation of Synthesis of Sparingly Soluble Compounds by Accurate MALDI-TOF Mass Spectrometry

User Benefits

- iMScope QT enables MALDI mass spectrometry of poorly soluble compounds that are difficult to analyze with LC/MS. - Using LCMS-9030, mass calibration with external standards enables accuracy to be maintained over a long period of time. - It is possible to confirm the accurate mass of poorly soluble synthetic products.

Introduction

The MALDI method is tolerant to the characteristics of the sample and thus, for example, can be used to perform mass spectrometry of poorly soluble compounds that are difficult to measure with LC-MS. For the analysis of less polar compounds, an ionizing agent is sometimes added to the sample and matrix to promote ionization. They must be mixed and dissolved, and then spotted and dried on a sample plate to form cocrystals. Therefore, it is necessary to be able to dissolve all components in the same solvent. On the other hand, for highly accurate mass measurement in MALDI-TOF MS analysis, it is desirable to perform mass calibration using internal standards. However, it takes time and effort to find calibrants with similar physical properties and similar molecular weight to the sample compound. The Q-TOF type mass spectrometer LCMS-9030 provides stable and accurate mass spectrometry over a long period of time with mass calibration using external standards. By attaching the iMScope QT with a MALDI ion source to the LCMS-9030 in place of the ESI, accurate mass measurement of poorly soluble compounds can be achieved.

June 28, 2022 GMT